Review





Similar Products

β actin  (Toyobo)
99
Toyobo β actin
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
β Actin, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β actin/product/Toyobo
Average 99 stars, based on 1 article reviews
β actin - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
β actin  (Vazyme Biotech Co)
99
Vazyme Biotech Co β actin
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
β Actin, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β actin/product/Vazyme Biotech Co
Average 99 stars, based on 1 article reviews
β actin - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
β actin gapdh  (Vazyme Biotech Co)
99
Vazyme Biotech Co β actin gapdh
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
β Actin Gapdh, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β actin gapdh/product/Vazyme Biotech Co
Average 99 stars, based on 1 article reviews
β actin gapdh - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
13222 ab 2714013 β actin wb  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc 13222 ab 2714013 β actin wb
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
13222 Ab 2714013 β Actin Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/13222 ab 2714013 β actin wb/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
13222 ab 2714013 β actin wb - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
actin b cybr green qpcr mix  (Thermo Fisher)
86
Thermo Fisher actin b cybr green qpcr mix
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
Actin B Cybr Green Qpcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin b cybr green qpcr mix/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
actin b cybr green qpcr mix - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
mixture β actin hrp linked antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mixture β actin hrp linked antibody
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
Mixture β Actin Hrp Linked Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mixture β actin hrp linked antibody/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
mixture β actin hrp linked antibody - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
nanjing vazyme biotech co β actin  (Vazyme Biotech Co)
99
Vazyme Biotech Co nanjing vazyme biotech co β actin
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
Nanjing Vazyme Biotech Co β Actin, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nanjing vazyme biotech co β actin/product/Vazyme Biotech Co
Average 99 stars, based on 1 article reviews
nanjing vazyme biotech co β actin - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
actin 8h10d10  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc actin 8h10d10
A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; <t>n=8).</t> <t>β-Actin</t> was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.
Actin 8h10d10, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actin 8h10d10/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
actin 8h10d10 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

Image Search Results


A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; n=8). β-Actin was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.

Journal: bioRxiv

Article Title: Epithelial Glutamate Retention Protects against Colitis

doi: 10.64898/2026.04.18.718419

Figure Lengend Snippet: A. mRNA Expression of SLC7A11 , encoding xCT, in human inflammatory bowel diseases. Publicly available data sets (GSE186507, GSE193677, GSE206285, GSE207465) were used for analysis. UC: ulcerative colitis, MES: Mayo endoscopic subscore, CD: Chrohn’s disease, SES-CD: Simple endoscopic score for CD. B. mRNA Expression of Slc7a11 , encoding xCT, in mouse colitic model induced by 2.5% DSS administration. Publicly available data sets (GSE131032) were used for analysis. C. RT-PCR for measuring mRNA expression of xCT in colon tissues of wild-type (WT) mice with or without 3% DSS treatment on day 6 (DSS – WT mice; n=8, DSS + WT mice; n=8). β-Actin was employed for normalization. Mean values of DSS – WT mice were set as 1. D-G. WT and xCT KO mice were treated with 3% DSS for 7 days and sacrificed on day 7 for analysis (n=14 per group). Body weight changes (D) , DAI score (E) , and colon length measurement and macroscopic observation of colon tissues (F) are shown. Histological score and HE staining of colon tissues are shown with scale bars corresponding to 1 mm and 100 μm for lower and higher magnifications, respectively ( G ). Data are shown as mean□±□SD from two independent experiments. H. Alcian blue staining of colon tissues from the mice sacrificed on day 7 after 3% DDS administration for detecting mucus. Untreated samples were obtained from mice given tap water. Representative micrographs are shown (n=2 per group). Scale bars correspond to 1 mm for lower magnification and 100 μm for higher magnification. I. Evaluation of intestinal permeability by FITC-dextran. FITC-dextran was quantified in serum with or without 3% DSS treatment on day 3 (DSS – WT mice; n=5, DSS – xCT KO mice; n=5, DSS + WT mice; n=10, DSS + xCT KO mice; n=11). Data are shown as mean□±□SD from three independent experiments. J. RT-PCR for measuring mRNA expression of cytokines, chemokines and a neutrophil marker, Ly6G, in colon tissues with 3% DSS treatment on day 6 (WT mice; n=8, xCT KO mice; n=8). β-Actin was employed for normalization. Mean values of WT mice were set as 1. Data are shown as mean□±□SD from two independent experiments. Data represent means ± standard deviation. A , B , and I were analyzed by one-way ANOVA. D and E were analyzed by two-way ANOVA. C , F , G , and J were analyzed by two-sided Student’s t-test. *p<0.05, **p<0.01, ****p<0.0001; ns, not significant.

Article Snippet: Real-time PCR was conducted on an Applied Biosystems 7300 Real-Time PCR System (7300 M, Thermo Fisher Scientific) using THUNDERBIRD® Probe qPCR Mix (#QPS-101, TOYOBO). β-Actin was employed for normalization.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Permeability, Marker, Standard Deviation

A. Detection of polyglutamylated proteins with or without glutamine in Caco-2 cells. Tubulin was detected as a loading control. Results from three independent experiments are shown. B. Measurement of transepithelial electrical resistance (TEER) of Caco-2 cell culture with or without glutamine. Data are shown as mean□±□SD from four independent experiments. C. RT-PCR to measure mRNA expression of IL-8 in HCT116 cells following TNFα stimulation with or without glutamine and SSZ (300 μM) (n=4, per group). β-Actin was employed for normalization. A mean value of samples with 4 mM glutamine without SSZ was set as one. Data are shown as mean□±□SD from four independent experiments. D. Knockdown efficiency of TTLL4 and TTLL5 in Caco-2 cells. Data are shown as mean□±□SD from six independent experiments. E. Detection of polyglutamylated proteins with or without knockdown of TTLL4 and/or TTLL5 in Caco-2 cells. Tubulin was detected as a loading control. Results from three independent experiments are shown. F. Measurement of TEER of Caco-2 cell culture with or without TTLL4 and TTLL5 double knockdown. Data are shown as mean ± SD from four independent experiments performed in duplicate. G. RT-PCR to measure mRNA expression of IL-8 in Caco-2 cells following TNFα stimulation with or without knockdown of TTLL4 and TTLL5 (n=3, per group). β-Actin was employed for normalization. A mean value of control siRNA-treated samples without TNFα stimulation was set as one. A representative result is shown from two independent experiments. Data represent means ± standard deviation. B and F were analyzed by two-way ANOVA. C , D and G were analyzed by one-way ANOVA. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

Journal: bioRxiv

Article Title: Epithelial Glutamate Retention Protects against Colitis

doi: 10.64898/2026.04.18.718419

Figure Lengend Snippet: A. Detection of polyglutamylated proteins with or without glutamine in Caco-2 cells. Tubulin was detected as a loading control. Results from three independent experiments are shown. B. Measurement of transepithelial electrical resistance (TEER) of Caco-2 cell culture with or without glutamine. Data are shown as mean□±□SD from four independent experiments. C. RT-PCR to measure mRNA expression of IL-8 in HCT116 cells following TNFα stimulation with or without glutamine and SSZ (300 μM) (n=4, per group). β-Actin was employed for normalization. A mean value of samples with 4 mM glutamine without SSZ was set as one. Data are shown as mean□±□SD from four independent experiments. D. Knockdown efficiency of TTLL4 and TTLL5 in Caco-2 cells. Data are shown as mean□±□SD from six independent experiments. E. Detection of polyglutamylated proteins with or without knockdown of TTLL4 and/or TTLL5 in Caco-2 cells. Tubulin was detected as a loading control. Results from three independent experiments are shown. F. Measurement of TEER of Caco-2 cell culture with or without TTLL4 and TTLL5 double knockdown. Data are shown as mean ± SD from four independent experiments performed in duplicate. G. RT-PCR to measure mRNA expression of IL-8 in Caco-2 cells following TNFα stimulation with or without knockdown of TTLL4 and TTLL5 (n=3, per group). β-Actin was employed for normalization. A mean value of control siRNA-treated samples without TNFα stimulation was set as one. A representative result is shown from two independent experiments. Data represent means ± standard deviation. B and F were analyzed by two-way ANOVA. C , D and G were analyzed by one-way ANOVA. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

Article Snippet: Real-time PCR was conducted on an Applied Biosystems 7300 Real-Time PCR System (7300 M, Thermo Fisher Scientific) using THUNDERBIRD® Probe qPCR Mix (#QPS-101, TOYOBO). β-Actin was employed for normalization.

Techniques: Control, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Expressing, Knockdown, Standard Deviation